02 Bacteria Culture

Bacterial culture

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• Blood: enriched (blood) and differential (blood pattern)

• Eosin methylene blue: selective (gm-) and differential (lactose fermenting)

Fastidious bacteria

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Blood Agar

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• pseudomonas is negative

Chocolate Agar

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.,

• staph aureus beta hemolysis first

VPN

_Aka thayer-martin media.,

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.,

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Bordet-Gengou agar

_Aka potato agar.,

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.,

• made from potatoes - high in starch

Loeffler's media

[_](Loeffler's media uses)

• selective for C. dphtheria.,

Tellurite plate

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• differential for c. diphtheria. Reduces tellurite to tellurium

• Gray black colonies.,

Lowenstein-Jensen

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Eaton's agar

[_](eaton's agar uses)

• culture for mycoplasma pneumonia.,

MacConkey's agar

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.,

Eosin-Methylene Blue

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.,

• EMB

Sorbitol MacConkey

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Buffered charcoal yeast extract

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• charcoal for legionella

• classic: nursing home with atypical pneumonia

Sabouraud's agar

[_](sabouraud's agar uses)

.,

Special growth requirements

Superoxide dismutase and catalase

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• O2 radical toxic to bacteria. Must be neutralized by aerobic organisms

Aerobes

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Anaerobes

[_](anaerobe bacteria location, causes disease when and where, what antibiotic doesn't work on them)..

• no O2 in abscess

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• bacteroides: appendicitis

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• Aspirate vomit

• Clindamycin for anaerobe above diaphragm (lung)

• Metronidazole for anaerobe below diaphragm

• Also can use cipro/quinolone for diverticulitis/appendicitis

Intracellular

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