06 PCR
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DNA polymerase requires primer
variant, quantitative
used for small amount of DNA curculating
HIV: RNA. Use reverse transcriptase to make copy of virus RNA: cDNA
binding = hybridization
put a piece of filter paper on top. DNA stick to it
DNA left complementary to probe
for characterizing genes
length of fragments unique to each particular gene
Restriction nuclease added to Gene A and Gene B: breaking up into different fragments
Compare unknown gene that break up to gene A or B
determine genotype
same technique for RNA
studies gene expression by finding mRNA corresponding to standard
use antibody to protein instead of probe
side and forward scatter
forward scatter based on cell size
side scatter based on granularity
lymphocytes: small, little granules
granulocytes: big, lots of granules
determine Hb F in maternal circulation = hemorrhage
good for detect proteins
add serum to one of the wells
plate designed so antigen stick to surface
wash away serum and left with antigens bound
direct: for every antigen test for, must have an antibody enzyme linked
indirect: more common
unbound antibody: free antibody from last slide
any HIV antibody in sample will bind to antigen in plate
like microarray, uses fluorescent
tell that gene is present on that chromosome
